Hi all! I've emphasized before how important and helpful it can be to have previous lab experience before going to graduate school. Well, recently, I've actually learned how that previous research experience can actually be a hindrance. Today I would like to post about what I'm going to call the "overly confident rotation student."
As you all know, I came into graduate school with what I consider to be a lot of research experience. I never thought of this as a disadvantage, until now. Why, do you ask? Well, let me lay some framework for why I feel this way...
All of my rotations thus far as a graduate student have been doing completely different things than I have ever done before in my previous experiences, for the most part. The first one consisted of a mouse procedure that I hadn't done before, tissue culture techniques that I'd never used, ELISA, etc. The second one was completely different too - lots of microscopy, immunostaining for parasites, etc. The only overlap in these were very general things like tissue culture, I have immunostained before (but never double fluorescent staining, etc.), I've done flow cytometry, and I've used a scope. But, the actual procedures were things I'd never done. And in both of these rotations, I've gotten As! Needless to say, Ellyn's head was getting a little inflated.
Then, a couple weeks ago, I started my third rotation, where I'm doing a cloning project and creating a deletion mutant. I was kind of excited about the cloning project! Finally, something I'm familiar with! Well, this familiarity kind of bit me in the ass. I have been setting up my PCRs, not really paying much attention to the nitty gritty details, because I've done PCR before! I know the dilutions that need to be done for the various components. I know all about melting temperatures and elongation temperatures and times. It clicks. I get it. I've done it. Well, I don't know about all the different PCR master mixes they use, and how they are not one and the same, and they all serve different functions. So, today I sit waiting to see if my third PCR works, because I was too much of an over-confident idiot to double check that I was using the right PCR mix in either of my previous 2 attempts. Damn it!
So - yes, I made a stupid mistake! But, the kicker is that I made a stupid mistake twice in a row, and could have easily fixed my mistake after the first incident. But, I didn't ask questions, and that's a problem! Fortunately, the issues I am still having with the PCR don't seem to be linked to the fact that I've used the wrong mastermix.
I told the post-doc that I'm working with that I admittedly was a little too confident about my abilities with PCR, because I've done it before, and will ask more questions in the future. I personally feel that is important. If you know you did something wrong, be mature and own up to it.
So, that's my lesson for the day! Don't be the overly confident rotation student! Even if you're pretty confident about what you're doing, ask clarifying questions just to be sure. The silliest thing you can do is make mistakes that are as avoidable as using the correct master mix!
Until next time,
I wanted to do this as little as possible - complain about grad school. But at some point everyone needs to realize that grad school is, at times, exactly the horror story they have heard. So, unfortunately, that is what my last week was.
From the blog - "What should I call grad school" - entitled "One Month into Graduate School"

Seriously - this is what my life has felt like the last few weeks?? What have I done?? Last week was such a Murphy's Law week, that describing it and re-experiencing it literally brings tears to my eyes. I re-hashed it to my dad yesterday, and I literally struggled to maintain composure. It was bad.
I'm a perfectionist. Admittedly. I enjoy succeeding, but really, who doesn't? I work my ass off though, and I really feel like I deserve the successes that I have. But, this week, it was like no matter how hard I worked, nothing ever seemed to go my way, and nothing ever seemed good enough.
In school - I had two exams last Thursday, in back to back classes. These weren't easy classes either. Epidemiology and Biostatistics. Biostatistics should have been so easy, really. But, unfortunately for our class, it was the first time he has ever taught the class, and we all proceeded to take the most poorly written exam I have ever taken in my life. He had a line of people in front of the class waiting to ask him questions for 90/120 minutes during the exam. After that brutality was over, a large group of us waited out in the hall for our Epidemiology exam to start. It started 10 minutes late, because of Biostatistics, and, of course, they didn't give us extra time. If they had, things may have been SO MUCH BETTER, because that exam was a BEAST!! 50 Questions in 80 minutes, and they were long questions!! It was all I could do to finish the exam.
I was so livid when I finished the exam. But, I pushed those thoughts out of my head and went to lab to complete a multitude of experiments that I had set up, and of course, everything was dead! Nothing worked - yada yada yada! Let's just say that you know it's a crappy day in lab when all 3 of your graduate students are at the bar at 2pm.
Within two days, it was no longer just an issue of crappy cells, slow-growing parasites, etc., but the bacterial contamination which had derailed me weeks before and had run rampant through the lab resurfaced again, and of course it did so in my cultures. REALLY??
From the blog - "What should I call grad school" - entitled "One Month into Graduate School"

Seriously - this is what my life has felt like the last few weeks?? What have I done?? Last week was such a Murphy's Law week, that describing it and re-experiencing it literally brings tears to my eyes. I re-hashed it to my dad yesterday, and I literally struggled to maintain composure. It was bad.
I'm a perfectionist. Admittedly. I enjoy succeeding, but really, who doesn't? I work my ass off though, and I really feel like I deserve the successes that I have. But, this week, it was like no matter how hard I worked, nothing ever seemed to go my way, and nothing ever seemed good enough.
In school - I had two exams last Thursday, in back to back classes. These weren't easy classes either. Epidemiology and Biostatistics. Biostatistics should have been so easy, really. But, unfortunately for our class, it was the first time he has ever taught the class, and we all proceeded to take the most poorly written exam I have ever taken in my life. He had a line of people in front of the class waiting to ask him questions for 90/120 minutes during the exam. After that brutality was over, a large group of us waited out in the hall for our Epidemiology exam to start. It started 10 minutes late, because of Biostatistics, and, of course, they didn't give us extra time. If they had, things may have been SO MUCH BETTER, because that exam was a BEAST!! 50 Questions in 80 minutes, and they were long questions!! It was all I could do to finish the exam.
I was so livid when I finished the exam. But, I pushed those thoughts out of my head and went to lab to complete a multitude of experiments that I had set up, and of course, everything was dead! Nothing worked - yada yada yada! Let's just say that you know it's a crappy day in lab when all 3 of your graduate students are at the bar at 2pm.
Within two days, it was no longer just an issue of crappy cells, slow-growing parasites, etc., but the bacterial contamination which had derailed me weeks before and had run rampant through the lab resurfaced again, and of course it did so in my cultures. REALLY??
To top it all off my "easy fix" of a car repair turned into a $100 electrical sensor issue, my garbage disposal broke, I randomly started bleeding on the bus from a magical gash on my finger that appeared out of nowhere, my sunglasses broke, and I ran out of ink in my printer and had to go spend more money on that.
It was a gem of a week - and it was one of those deals where I honestly started questioning myself. I hate seeming incompetent. Especially when I know I am competent. Fortunately, everyone in the lab is super understanding and awesome about all the setbacks I've had during my rotation, and somehow my PI is still really impressed with the data that I've gotten. But, unforunately, grades don't work that way. Professors aren't going to pass you because they're understanding. And that just sucks. I'm feeling better about Biostats. But Epidemiology is a different story. I'm terrified that this bad test is going to define whether or not I'm even here next semester. If I don't pass, Epi what happens? I literally do think that it went that badly, and I'm freaking out thinking about what it will mean for all the work I put in to get here.
I never expected grad school to be all sunshine and roses. I knew better coming in. I'd worked with grad students before and I knew that it was hell at times. But when it's this bad, how in the world do you possibly pick yourself and keep going?
The trick to survival - I already know it! It's your friends!
The trick to survival - I already know it! It's your friends!
My friends and I made a pact at the beginning of the semester. If we quit, we quit together. If one person wants to quit, they can't until everyone is ready to quit. :-) Seems kind of negative and pessimistic, but it's a good thing to keep us going. There's a couple of us in this pact, and we're all in different departments (mostly). So, the likelihood that we will all be in hell, unable to see the light at the end of the tunnel, all at the same time...slim to none. Hopefully!
So, that's my tragic little story right now. It's so not tragic - I know. I probably should not be complaining, but hey - everyone needed to know that my first semester in graduate school isn't all great life lessons and good times. At some point I was going to have to introduce you to the reality of what graduate school will be like, probably more often than not.
I want to talk about the importance of pursuing research opportunities for science majors, especially those who are hoping to go on to graduate school.
It was more than enough. I got interviews at 6 of the 9 schools I applied to. I was giddy, ecstatic, slap happy when I got the phone call from Michigan saying I'd gotten an interview! They were the first people to call and offer me an interview, and from there the calls kept rolling in. But, with the excitement came nerves. What would happen during interviews? What would I get asked? What should I wear? Would I really have to sell myself? Would they already know my application or would I have to reiterate it to them? It was an unbelievable whirlwind of questions. So I prepared the only way I knew how - as if I were prepping for a presentation in undergrad. I googled potential interview questions. I wrote out answers. I memorized my answers. I recorded myself asking the questions and then played back the answers as if I were the interviewer. I even had a good friend of mine who was currently enrolled in a PhD program practice interview me (she's the best!!). I know. I'm an overachiever. And, as it turns out, I massively over-prepared. Interviews were a breeze. It was more stressful practicing with my friend than it was actually interviewing. I was told by one interviewer that I had "many fans on the review board," another said that he was "blown away" by my experience, and another said "I would admit you right now if I had that power."
I'm going to do this the only way that makes sense to me; by telling my story - how I got to this point in my career/education, and how scientific research has done nothing but benefit me ...
When I was in 9th grade I had a really legit biology class. My teacher had gotten a grant from like Bill Gates or some big shot like that to introduce higher level science into her biology classroom. In this class, I used a pipet for the first time, I ran my first gel electrophoresis, we dissected TONS of things (squid, frogs, worms, etc.), and, most importantly, we watched the movie Outbreak. Now, all you scientists out there reading this might be scoffing, laughing, or shaking your head. "Really? Outbreak? What a terrible movie!!" Which, ya, now that I'm older and knowledgeable in the ways of science, I understand that. But to an inexperienced, naive little junior high schooler, this moment was legendary! I was so enthralled by this cheesy Hollywood melodrama. It was by far the coolest thing I'd ever seen.
So, I started devouring everything I could get my hands. I started reading up on microbiology, I printed out a picture of the Ebola virus which hung out in my desk. SUCH. A. NERD! I know. And, when it came time for me to enroll in college, I knew exactly what I wanted to do. I limited the colleges where I applied to only those with Microbiology degrees. I knew what I wanted to do. There was no point in wasting time with something else. I'm an oddball in that sense. I always new what I wanted.
When I started college, I knew about undergraduate research. I wanted to check it out, but I really wanted to give myself an opportunity to settle into college before I took on a job too. But, when my roommate informed me about a paid undergraduate research assistant position advertised in our Honors college newsletter, I figured, why not? It was in a reproductive biology lab, but I didn't mind. I just wanted to start. I wanted to experience research and decide if I liked it. And this was by far the best decision I have ever made for my education.
I spent the next 4 years putting in 10-20 hours a week in the lab. I came in before, between, and after classes. I was lucky, though. The lab I was in was very well funded. So well funded that he was able to pay his undergraduates. This is not the norm. Normally, undergraduate positions start as being volunteer positions. Oftentimes, these volunteers would simply wash dishes, autoclave, fill pipet tips, among other tedious and mundane tasks in the lab. Sometimes, they would be allowed to help with some of the less technically challenging work in the lab. It really depends upon the lab, but more often than not positions are not paid. Sometimes, though, these undergrads could be "promoted," if you will, to paid positions. But, again, this really depends upon the lab. But, I lucked out and I was paid for my work.
When I was a freshman, I mainly washed dishes, perpetuated the rat colony (definitely one of the least glamorous parts of the job), made buffers, and occasionally I would set up PCRs and run gels. Eventually though, because I had been in the lab so long and was so familiar, I was allowed to start more technical work on one of the projects. This project would become my undergraduate thesis for the honors college of which I was a part, and this same research would be published in a scientific journal, and I would be an author. A second author.
This is why you should start early! I got in on the ground floor in this lab, and by the time I left I felt like an integral part of the team, and I was. I was essentially managing all of the younger undergrads. I worked independently on all of my projects and I learned so many new skills, at which I was competent to perform and troubleshoot on my own. This would probably have been enough to set me on a blazing path toward graduate school. But, I wasn't sure what I wanted at the time. PhD? MD? MD/PhD? Microbiology? Immunology? Infectious diseases? Pathology? Pathogenesis? The opportunities seemed vast, endless, and my normal decisiveness was failing me. I was confused. What would be the best option for me?
So I took a couple years off from school after graduation. I applied and got a job as a research technician in a gene therapy lab - a job that I never would've gotten if I hadn't had the research experience that I accumulated as an undergrad. Literally! I found out toward the end of my time in this next lab that one of the graduate students had applied for the same position and hadn't gotten it because she was told that there was an applicant with more experience - me!
Now, this brings me to applying to graduate school. About 1.5 years into my job as a research technician, I decided to apply to graduate school. An MD wasn't for me. I had no interest in working with patients. My place was at the bench, making the discoveries. So, I started applying to PhD programs all over the country. I even looked internationally for a bit. I wanted to get out of dodge and really experience something different from the tiny college town I had lived in for so long. I spent a long time researching programs leading up to the actual submissions. If I had learned anything from the time spent working with graduate students in these couple labs it was that the program isn't as important as the research. Find research and an environment that suits you, not course work that suits you, because you will only be taking classes for a couple years and your research will take up the bulk of your time.
I applied to vastly too many schools in retrospect. Emory University. University of Michigan. Stanford University. UC-Berkeley. Washington University in St. Louis. UNC-Chapel Hill. Indiana University. Northwestern University. Oregon Health and Sciences University. But, I was unbelievably paranoid about my application. I was 77th percentile on my GRE. I graduated Cum Laude, but I wasn't a stellar student. I didn't go to a highly regarded school for undergrad, which I really thought would work against me. I spent a lot of time on my essays, but what if they didn't like them? The only thing that I knew I had working toward my advantage was I was applying with almost 6 years of research experience under my belt. I hope that would be enough to make me stand out.
It was more than enough. I got interviews at 6 of the 9 schools I applied to. I was giddy, ecstatic, slap happy when I got the phone call from Michigan saying I'd gotten an interview! They were the first people to call and offer me an interview, and from there the calls kept rolling in. But, with the excitement came nerves. What would happen during interviews? What would I get asked? What should I wear? Would I really have to sell myself? Would they already know my application or would I have to reiterate it to them? It was an unbelievable whirlwind of questions. So I prepared the only way I knew how - as if I were prepping for a presentation in undergrad. I googled potential interview questions. I wrote out answers. I memorized my answers. I recorded myself asking the questions and then played back the answers as if I were the interviewer. I even had a good friend of mine who was currently enrolled in a PhD program practice interview me (she's the best!!). I know. I'm an overachiever. And, as it turns out, I massively over-prepared. Interviews were a breeze. It was more stressful practicing with my friend than it was actually interviewing. I was told by one interviewer that I had "many fans on the review board," another said that he was "blown away" by my experience, and another said "I would admit you right now if I had that power."
After everything, I got into every school I interviewed at. I was actually sitting at the gate in the Atlanta airport waiting to de-board the plan on my way to my 3rd interview when I got the call with my first official acceptance. From that day forward, the offers rolled in from each of my schools. Some of the top Microbiology programs in the nation and I got into 6 of them. I had my pick of the litter. At that point, it came down to the best fit for me. Not the best program on paper or even the best research fit for me. Where could I see myself living, integrating, and blossoming as a researcher over the next 4-7 years? Though I stressed and freaked out over it for a period of time, the choice really was easy. I knew where I wanted to be in my gut. I knew what the best place was for me and now I'm living it.
Getting research experience set me up so well for my future. Not only am I steps ahead of my first year colleagues in many cases, but I have a much better feel for the kind of research I like. I know the lab atmosphere. I know what I'm comfortable with. I'm experienced in lab notebook keeping. I'm experienced in designing an experiment, troubleshooting, etc. Having experience in that is so useful, and though it by no means makes me an expert, it makes it so much easier to dive into graduate school.
So, that's my story. If you have any questions about research, where to look for job postings, graduate school applications, interviews, choosing the right fit for you, let me know! If you want me to cover a specific topic, let me know! I want to help you in any way that I can! I'll be posting periodically on aspects of what I have experienced -- how to choose an undergrad lab, etiquette of an undergrad researcher, applying to grad school, interviews, etc. later on.
Until next time!






